Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
Mais filtros








Base de dados
Intervalo de ano de publicação
1.
Sci Rep ; 13(1): 10664, 2023 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-37393322

RESUMO

Hydrological disasters, such as floods, can have dire consequences for human societies. Historical information plays a key role in detecting whether particular types of hydrological disasters have increased in frequency and/or magnitude and, if so, they are more likely attributable to natural or human-induced climatic and other environmental changes. The identification of regions with similar flood conditions is essential for the analysis of regional flooding regimes. To this end, we here present the longest existing flood reconstruction for the Eastern Liguria Area (ELA) in northwestern Italy, covering 1582 to 2022 CE, which offers a case study representative of the central Mediterranean region. An Annual Flood Intensification Index was developed to transform the historical data into a continuous annual hydrological time-series contained by a homogeneous data structure for the study-area. We found two change-points (trend breaks) in the reconstructed time-series, in 1787 and 1967, with only occasional heavy floods comparable to present-day disasters occurring before the first change-point, and an increasing intensification of floods after the second change-point up to the present day. The recent intensification of flooding in the ELA, associated with changes in land use and land cover, also appears to coincide with phases in which hydrological hazards have become more changeable and extreme in disaster-affected areas. This is evidenced by river basin responses to human-induced disturbances.


Assuntos
Desastres , Terapia Implosiva , Humanos , Inundações , Hidrologia , Itália
2.
Environ Sci Technol ; 56(18): 13485-13498, 2022 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-36052879

RESUMO

There is a growing realization that the complexity of model ensemble studies depends not only on the models used but also on the experience and approach used by modelers to calibrate and validate results, which remain a source of uncertainty. Here, we applied a multi-criteria decision-making method to investigate the rationale applied by modelers in a model ensemble study where 12 process-based different biogeochemical model types were compared across five successive calibration stages. The modelers shared a common level of agreement about the importance of the variables used to initialize their models for calibration. However, we found inconsistency among modelers when judging the importance of input variables across different calibration stages. The level of subjective weighting attributed by modelers to calibration data decreased sequentially as the extent and number of variables provided increased. In this context, the perceived importance attributed to variables such as the fertilization rate, irrigation regime, soil texture, pH, and initial levels of soil organic carbon and nitrogen stocks was statistically different when classified according to model types. The importance attributed to input variables such as experimental duration, gross primary production, and net ecosystem exchange varied significantly according to the length of the modeler's experience. We argue that the gradual access to input data across the five calibration stages negatively influenced the consistency of the interpretations made by the modelers, with cognitive bias in "trial-and-error" calibration routines. Our study highlights that overlooking human and social attributes is critical in the outcomes of modeling and model intercomparison studies. While complexity of the processes captured in the model algorithms and parameterization is important, we contend that (1) the modeler's assumptions on the extent to which parameters should be altered and (2) modeler perceptions of the importance of model parameters are just as critical in obtaining a quality model calibration as numerical or analytical details.


Assuntos
Carbono , Solo , Ecossistema , Humanos , Nitrogênio , Incerteza
3.
PLoS One ; 17(8): e0272161, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36001546

RESUMO

BACKGROUND: Advances in climate change research contribute to improved forecasts of hydrological extremes with potentially severe impacts on human societies and natural landscapes. Rainfall erosivity density (RED), i.e. rainfall erosivity (MJ mm hm-2 h-1 yr-1) per rainfall unit (mm), is a measure of rainstorm aggressiveness and a proxy indicator of damaging hydrological events. METHODS AND FINDINGS: Here, using downscaled RED data from 3,625 raingauges worldwide and log-normal ordinary kriging with probability mapping, we identify damaging hydrological hazard-prone areas that exceed warning and alert thresholds (1.5 and 3.0 MJ hm-2 h-1, respectively). Applying exceedance probabilities in a geographical information system shows that, under current climate conditions, hazard-prone areas exceeding a 50% probability cover ~31% and ~19% of the world's land at warning and alert states, respectively. CONCLUSION: RED is identified as a key driver behind the spatial growth of environmental disruption worldwide (with tropical Latin America, South Africa, India and the Indian Archipelago most affected).


Assuntos
Desastres , Chuva , Sistemas de Informação Geográfica , Humanos , Hidrologia , Solo
4.
PLoS One ; 17(1): e0262132, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35061741

RESUMO

BACKGROUND: Rainfall and other climatic agents are the main triggers of soil erosion in the Mediterranean region, where they have the potential to increase discharge and sediment transport and cause long-term changes in the river system. For the Magra River Basin (MRB), located in the upper Tyrrhenian coast of Italy, we estimated changes in net erosion as a function of the geographical characteristics of the basin, the seasonal distribution of precipitation, and the vegetation cover. METHODS AND FINDINGS: Based on rainfall erosivity and surface flow and transport sub-models, we developed a simplified model to assess basin-wide sediment yields on a monthly basis by upscaling the point rainfall input. Our calibration dataset of monthly data (Mg km-2 month-1, available for the years 1961 and 1963-1969) revealed that our model satisfactorily reproduces the net soil erosion in the study area (R2 = 0.81). For the period 1950-2020, the reconstruction of an annually aggregated time-series of monthly net erosion data (297 Mg km-2 yr-1 on average) indicated a moderate decline in sediment yield after 1999. This is part of a long-term downward trend, which highlights the role played by land-use changes and reforestation of the mountainous areas of the basin. CONCLUSION: This study shows the environmental history and dynamics of the basin, and thus the varying sensitivity of hydrological processes and their perturbations. Relying on a few climatic variables as reported from a single representative basin location, it provides an interpretation of empirically determined factors that shape active erosional landscapes. In particular, we showed that the most recent extreme storms associated with sediment yield have been characterised by lower cumulative rainfall, indicating a greater propensity for the basin to produce sediment more discontinuously over time.


Assuntos
Monitoramento Ambiental , Erosão do Solo , Mudança Climática , História do Século XX , Humanos , Itália , Chuva , Erosão do Solo/história , Fatores de Tempo
5.
Sci Rep ; 11(1): 20518, 2021 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-34654846

RESUMO

Rainfall erosivity drives damaging hydrological events with significant environmental and socio-economic impacts. This study presents the world's hitherto longest time-series of annual rainfall erosivity (725-2019 CE), one from the Tiber River Basin (TRB), a fluvial valley in central Italy in which the city of Rome is located. A historical perspective of erosive floods in the TRB is provided employing a rainfall erosivity model based on documentary data, calibrated against a sample (1923-1964) of actual measurement data. Estimates show a notable rainfall erosivity, and increasing variability, during the Little Ice Age (here, ~ 1250-1849), especially after c. 1495. During the sixteenth century, erosive forcing peaked at > 3500 MJ mm hm-2 h-1 yr-1 in 1590, with values > 2500 MJ mm hm-2 h-1 yr-1 in 1519 and 1566. Rainfall erosivity continued into the Current Warm Period (since ~ 1850), reaching a maximum of ~ 3000 MJ mm hm-2 h-1 yr-1 in the 1940s. More recently, erosive forcing has attenuated, though remains critically high (e.g., 2087 and 2008 MJ mm hm-2 h-1 yr-1 in 1992 and 2005, respectively). Comparison of the results with sediment production (1934-1973) confirms the model's ability to predict geomorphological effects in the TRB, and reflects the role of North Atlantic circulation dynamics in central Italian river basins.

6.
Glob Chang Biol ; 27(4): 904-928, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33159712

RESUMO

Simulation models represent soil organic carbon (SOC) dynamics in global carbon (C) cycle scenarios to support climate-change studies. It is imperative to increase confidence in long-term predictions of SOC dynamics by reducing the uncertainty in model estimates. We evaluated SOC simulated from an ensemble of 26 process-based C models by comparing simulations to experimental data from seven long-term bare-fallow (vegetation-free) plots at six sites: Denmark (two sites), France, Russia, Sweden and the United Kingdom. The decay of SOC in these plots has been monitored for decades since the last inputs of plant material, providing the opportunity to test decomposition without the continuous input of new organic material. The models were run independently over multi-year simulation periods (from 28 to 80 years) in a blind test with no calibration (Bln) and with the following three calibration scenarios, each providing different levels of information and/or allowing different levels of model fitting: (a) calibrating decomposition parameters separately at each experimental site (Spe); (b) using a generic, knowledge-based, parameterization applicable in the Central European region (Gen); and (c) using a combination of both (a) and (b) strategies (Mix). We addressed uncertainties from different modelling approaches with or without spin-up initialization of SOC. Changes in the multi-model median (MMM) of SOC were used as descriptors of the ensemble performance. On average across sites, Gen proved adequate in describing changes in SOC, with MMM equal to average SOC (and standard deviation) of 39.2 (±15.5) Mg C/ha compared to the observed mean of 36.0 (±19.7) Mg C/ha (last observed year), indicating sufficiently reliable SOC estimates. Moving to Mix (37.5 ± 16.7 Mg C/ha) and Spe (36.8 ± 19.8 Mg C/ha) provided only marginal gains in accuracy, but modellers would need to apply more knowledge and a greater calibration effort than in Gen, thereby limiting the wider applicability of models.


Assuntos
Carbono , Solo , Agricultura , Carbono/análise , França , Federação Russa , Suécia , Incerteza , Reino Unido
7.
Sci Rep ; 10(1): 22062, 2020 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-33328541

RESUMO

Rainfall erosivity and its derivative, erosivity density (ED, i.e., the erosivity per unit of rain), is a main driver of considerable environmental damages and economic losses worldwide. This study is the first to investigate the interannual variability, and return periods, of both rainfall erosivity and ED over the Mediterranean for the period 1680-2019. By capturing the relationship between seasonal rainfall, its variability, and recorded hydrological extremes in documentary data consistent with a sample (1981-2015) of detailed Revised Universal Soil Loss Erosion-based data, we show a noticeable decreasing trend of rainfall erosivity since about 1838. However, the 30-year return period of ED values indicates a positive long-term trend, in tandem with the resurgence of very wet days (> 95th percentile) and the erosive activity of rains during the past two decades. A possible fingerprint of recent warming is the occurrence of prolonged wet spells in apparently more erratic and unexpected ways.

8.
Sci Rep ; 10(1): 9982, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32546705

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

9.
Sci Rep ; 9(1): 9963, 2019 07 10.
Artigo em Inglês | MEDLINE | ID: mdl-31292466

RESUMO

Damaging hydrological events are extreme phenomena with potentially severe impacts on human societies. Here, we present the hitherto longest reconstruction of damaging hydrological events for Italy, and for the whole Mediterranean region, revealing 674 such events over the period 800-2017. For any given year, we established a severity index based on information in historical documentary records, facilitating the transformation of the data into a continuous time-series. Episodes of hydrological extremes disrupted ecosystems during the more severe events by changing landforms. The frequency and severity of damaging hydrological events across Italy were likely influenced by the mode of the Atlantic Multidecadal Variability (AMV), with relatively few events during the warm Medieval Climate Anomaly dominated by a positive phase of the AMV. More frequent and heavier storms prevailed during the cold Little Ice Age, dominated by a more negative phase of the AMV. Since the mid-19th century, a decreasing occurrence of exceptional hydrological events is evident, especially during the most recent decades, but this decrease is not yet unprecedented in the context of the past twelve centuries.

10.
Sci Rep ; 9(1): 9258, 2019 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-31239485

RESUMO

Climate change studies involve complex processes translating coarse climate change projections in locally meaningful terms. We analysed the behaviour of weather generators while downscaling precipitation and air temperature data. With multiple climate indices and alternative weather generators, we directly quantified the uncertainty associated with using weather generators when site specific downscaling is performed. We extracted the influence of weather generators on climate variability at local scale and the uncertainty that could affect impact assessment. For that, we first designed the downscaling experiments with three weather generators (CLIMAK, LARS-WG, WeaGETS) to interpret future projections. Then we assessed the impacts of estimated changes of precipitation and air temperature for a sample of 15 sites worldwide using a rice yield model and an extended set of climate metrics. We demonstrated that the choice of a weather generator in the downscaling process may have a higher impact on crop yield estimates than the climate scenario adopted. Should they be confirmed, these results would indicate that widely accepted outcomes of climate change studies using this downscaling technique need reconsideration.

11.
J Exp Bot ; 70(9): 2587-2604, 2019 04 29.
Artigo em Inglês | MEDLINE | ID: mdl-30753587

RESUMO

Agricultural systems models are complex and tend to be over-parameterized with respect to observational datasets. Practical identifiability analysis based on local sensitivity analysis has proved effective in investigating identifiable parameter sets in environmental models, but has not been applied to agricultural systems models. Here, we demonstrate that identifiability analysis improves experimental design to ensure independent parameter estimation for yield and quality outputs of a complex grassland model. The Pasture Simulation model (PaSim) was used to demonstrate the effectiveness of practical identifiability analysis in designing experiments and measurement protocols within phenotyping experiments with perennial ryegrass. Virtual experiments were designed combining three factors: frequency of measurements, duration of the experiment. and location of trials. Our results demonstrate that (i) PaSim provides sufficient detail in terms of simulating biomass yield and quality of perennial ryegrass for use in breeding, (ii) typical breeding trials are insufficient to parameterize all influential parameters, (iii) the frequency of measurements is more important than the number of growing seasons to improve the identifiability of PaSim parameters, and (iv) identifiability analysis provides a sound approach for optimizing the design of multi-location trials. Practical identifiability analysis can play an important role in ensuring proper exploitation of phenotypic data and cost-effective multi-location experimental designs. Considering the growing importance of simulation models, this study supports the design of experiments and measurement protocols in the phenotyping networks that have recently been organized.


Assuntos
Lolium/crescimento & desenvolvimento , Lolium/fisiologia , Cruzamento , Pradaria , Modelos Biológicos , Fenótipo
12.
Land Degrad Dev ; 29(8): 2378-2389, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30393451

RESUMO

Soils are vital for supporting food security and other ecosystem services. Climate change can affect soil functions both directly and indirectly. Direct effects include temperature, precipitation, and moisture regime changes. Indirect effects include those that are induced by adaptations such as irrigation, crop rotation changes, and tillage practices. Although extensive knowledge is available on the direct effects, an understanding of the indirect effects of agricultural adaptation options is less complete. A review of 20 agricultural adaptation case-studies across Europe was conducted to assess implications to soil threats and soil functions and the link to the Sustainable Development Goals (SDGs). The major findings are as follows: (a) adaptation options reflect local conditions; (b) reduced soil erosion threats and increased soil organic carbon are expected, although compaction may increase in some areas; (c) most adaptation options are anticipated to improve the soil functions of food and biomass production, soil organic carbon storage, and storing, filtering, transforming, and recycling capacities, whereas possible implications for soil biodiversity are largely unknown; and (d) the linkage between soil functions and the SDGs implies improvements to SDG 2 (achieving food security and promoting sustainable agriculture) and SDG 13 (taking action on climate change), whereas the relationship to SDG 15 (using terrestrial ecosystems sustainably) is largely unknown. The conclusion is drawn that agricultural adaptation options, even when focused on increasing yields, have the potential to outweigh the negative direct effects of climate change on soil degradation in many European regions.

13.
Sci Total Environ ; 642: 292-306, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-29902627

RESUMO

Simulation models quantify the impacts on carbon (C) and nitrogen (N) cycling in grassland systems caused by changes in management practices. To support agricultural policies, it is however important to contrast the responses of alternative models, which can differ greatly in their treatment of key processes and in their response to management. We applied eight biogeochemical models at five grassland sites (in France, New Zealand, Switzerland, United Kingdom and United States) to compare the sensitivity of modelled C and N fluxes to changes in the density of grazing animals (from 100% to 50% of the original livestock densities), also in combination with decreasing N fertilization levels (reduced to zero from the initial levels). Simulated multi-model median values indicated that input reduction would lead to an increase in the C sink strength (negative net ecosystem C exchange) in intensive grazing systems: -64 ±â€¯74 g C m-2 yr-1 (animal density reduction) and -81 ±â€¯74 g C m-2 yr-1 (N and animal density reduction), against the baseline of -30.5 ±â€¯69.5 g C m-2 yr-1 (LSU [livestock units] ≥ 0.76 ha-1 yr-1). Simulations also indicated a strong effect of N fertilizer reduction on N fluxes, e.g. N2O-N emissions decreased from 0.34 ±â€¯0.22 (baseline) to 0.1 ±â€¯0.05 g N m-2 yr-1 (no N fertilization). Simulated decline in grazing intensity had only limited impact on the N balance. The simulated pattern of enteric methane emissions was dominated by high model-to-model variability. The reduction in simulated offtake (animal intake + cut biomass) led to a doubling in net primary production per animal (increased by 11.6 ±â€¯8.1 t C LSU-1 yr-1 across sites). The highest N2O-N intensities (N2O-N/offtake) were simulated at mown and extensively grazed arid sites. We show the possibility of using grassland models to determine sound mitigation practices while quantifying the uncertainties associated with the simulated outputs.

14.
Glob Chang Biol ; 24(2): e603-e616, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29080301

RESUMO

Simulation models are extensively used to predict agricultural productivity and greenhouse gas emissions. However, the uncertainties of (reduced) model ensemble simulations have not been assessed systematically for variables affecting food security and climate change mitigation, within multi-species agricultural contexts. We report an international model comparison and benchmarking exercise, showing the potential of multi-model ensembles to predict productivity and nitrous oxide (N2 O) emissions for wheat, maize, rice and temperate grasslands. Using a multi-stage modelling protocol, from blind simulations (stage 1) to partial (stages 2-4) and full calibration (stage 5), 24 process-based biogeochemical models were assessed individually or as an ensemble against long-term experimental data from four temperate grassland and five arable crop rotation sites spanning four continents. Comparisons were performed by reference to the experimental uncertainties of observed yields and N2 O emissions. Results showed that across sites and crop/grassland types, 23%-40% of the uncalibrated individual models were within two standard deviations (SD) of observed yields, while 42 (rice) to 96% (grasslands) of the models were within 1 SD of observed N2 O emissions. At stage 1, ensembles formed by the three lowest prediction model errors predicted both yields and N2 O emissions within experimental uncertainties for 44% and 33% of the crop and grassland growth cycles, respectively. Partial model calibration (stages 2-4) markedly reduced prediction errors of the full model ensemble E-median for crop grain yields (from 36% at stage 1 down to 4% on average) and grassland productivity (from 44% to 27%) and to a lesser and more variable extent for N2 O emissions. Yield-scaled N2 O emissions (N2 O emissions divided by crop yields) were ranked accurately by three-model ensembles across crop species and field sites. The potential of using process-based model ensembles to predict jointly productivity and N2 O emissions at field scale is discussed.


Assuntos
Agricultura/métodos , Produtos Agrícolas/fisiologia , Modelos Biológicos , Óxido Nitroso/metabolismo , Simulação por Computador , Abastecimento de Alimentos , Incerteza
15.
Sci Total Environ ; 598: 445-470, 2017 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-28454025

RESUMO

Biogeochemical simulation models are important tools for describing and quantifying the contribution of agricultural systems to C sequestration and GHG source/sink status. The abundance of simulation tools developed over recent decades, however, creates a difficulty because predictions from different models show large variability. Discrepancies between the conclusions of different modelling studies are often ascribed to differences in the physical and biogeochemical processes incorporated in equations of C and N cycles and their interactions. Here we review the literature to determine the state-of-the-art in modelling agricultural (crop and grassland) systems. In order to carry out this study, we selected the range of biogeochemical models used by the CN-MIP consortium of FACCE-JPI (http://www.faccejpi.com): APSIM, CERES-EGC, DayCent, DNDC, DSSAT, EPIC, PaSim, RothC and STICS. In our analysis, these models were assessed for the quality and comprehensiveness of underlying processes related to pedo-climatic conditions and management practices, but also with respect to time and space of application, and for their accuracy in multiple contexts. Overall, it emerged that there is a possible impact of ill-defined pedo-climatic conditions in the unsatisfactory performance of the models (46.2%), followed by limitations in the algorithms simulating the effects of management practices (33.1%). The multiplicity of scales in both time and space is a fundamental feature, which explains the remaining weaknesses (i.e. 20.7%). Innovative aspects have been identified for future development of C and N models. They include the explicit representation of soil microbial biomass to drive soil organic matter turnover, the effect of N shortage on SOM decomposition, the improvements related to the production and consumption of gases and an adequate simulations of gas transport in soil. On these bases, the assessment of trends and gaps in the modelling approaches currently employed to represent biogeochemical cycles in crop and grassland systems appears an essential step for future research.

16.
Sci Total Environ ; 571: 50-8, 2016 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-27459253

RESUMO

The boreal forest of the northern hemisphere represents one of the world's largest ecozones and contains nearly one third of the world's intact forests and terrestrially stored carbon. Long-term variations in temperature and precipitation have been implied in altering carbon cycling in forest soils, including increased fluxes to receiving waters. In this study, we use a simple hydrologic model and a 40-year dataset (1971-2010) of dissolved organic carbon (DOC) from two pristine boreal lakes (ELA, Canada) to examine the interactions between precipitation and landscape-scale controls of DOC production and export from forest catchments to surface waters. Our results indicate that a simplified hydrologically-based conceptual model can enable the long-term temporal patterns of DOC fluxes to be captured within boreal landscapes. Reconstructed DOC exports from forested catchments in the period 1901-2012 follow largely a sinusoidal pattern, with a period of about 37years and are tightly linked to multi-decadal patterns of precipitation. By combining our model with long-term precipitation estimates, we found no evidence of increasing DOC transport or in-lake concentrations through the 20th century.

17.
Sci Total Environ ; 566-567: 851-864, 2016 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-27259038

RESUMO

Grassland-based ruminant production systems are integral to sustainable food production in Europe, converting plant materials indigestible to humans into nutritious food, while providing a range of environmental and cultural benefits. Climate change poses significant challenges for such systems, their productivity and the wider benefits they supply. In this context, grassland models have an important role in predicting and understanding the impacts of climate change on grassland systems, and assessing the efficacy of potential adaptation and mitigation strategies. In order to identify the key challenges for European grassland modelling under climate change, modellers and researchers from across Europe were consulted via workshop and questionnaire. Participants identified fifteen challenges and considered the current state of modelling and priorities for future research in relation to each. A review of literature was undertaken to corroborate and enrich the information provided during the horizon scanning activities. Challenges were in four categories relating to: 1) the direct and indirect effects of climate change on the sward 2) climate change effects on grassland systems outputs 3) mediation of climate change impacts by site, system and management and 4) cross-cutting methodological issues. While research priorities differed between challenges, an underlying theme was the need for accessible, shared inventories of models, approaches and data, as a resource for stakeholders and to stimulate new research. Developing grassland models to effectively support efforts to tackle climate change impacts, while increasing productivity and enhancing ecosystem services, will require engagement with stakeholders and policy-makers, as well as modellers and experimental researchers across many disciplines. The challenges and priorities identified are intended to be a resource 1) for grassland modellers and experimental researchers, to stimulate the development of new research directions and collaborative opportunities, and 2) for policy-makers involved in shaping the research agenda for European grassland modelling under climate change.

18.
BMC Biotechnol ; 10: 55, 2010 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-20687918

RESUMO

BACKGROUND: The modular approach to analysis of genetically modified organisms (GMOs) relies on the independence of the modules combined (i.e. DNA extraction and GM quantification). The validity of this assumption has to be proved on the basis of specific performance criteria. RESULTS: An experiment was conducted using, as a reference, the validated quantitative real-time polymerase chain reaction (PCR) module for detection of glyphosate-tolerant Roundup Ready(R) GM soybean (RRS). Different DNA extraction modules (CTAB, Wizard and Dellaporta), were used to extract DNA from different food/feed matrices (feed, biscuit and certified reference material [CRM 1%]) containing the target of the real-time PCR module used for validation. Purity and structural integrity (absence of inhibition) were used as basic criteria that a DNA extraction module must satisfy in order to provide suitable template DNA for quantitative real-time (RT) PCR-based GMO analysis. When performance criteria were applied (removal of non-compliant DNA extracts), the independence of GMO quantification from the extraction method and matrix was statistically proved, except in the case of Wizard applied to biscuit. A fuzzy logic-based procedure also confirmed the relatively poor performance of the Wizard/biscuit combination. CONCLUSIONS: For RRS, this study recognises that modularity can be generally accepted, with the limitation of avoiding combining highly processed material (i.e. biscuit) with a magnetic-beads system (i.e. Wizard).


Assuntos
Análise de Alimentos/métodos , Glycine max/genética , Plantas Geneticamente Modificadas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Ração Animal/análise , DNA de Plantas/análise , DNA de Plantas/isolamento & purificação , Alimentos Geneticamente Modificados , Projetos Piloto
19.
Transgenic Res ; 19(1): 57-65, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19533405

RESUMO

This paper illustrates the advantages that a fuzzy-based aggregation method could bring into the validation of a multiplex method for GMO detection (DualChip GMO kit, Eppendorf). Guidelines for validation of chemical, bio-chemical, pharmaceutical and genetic methods have been developed and ad hoc validation statistics are available and routinely used, for in-house and inter-laboratory testing, and decision-making. Fuzzy logic allows summarising the information obtained by independent validation statistics into one synthetic indicator of overall method performance. The microarray technology, introduced for simultaneous identification of multiple GMOs, poses specific validation issues (patterns of performance for a variety of GMOs at different concentrations). A fuzzy-based indicator for overall evaluation is illustrated in this paper, and applied to validation data for different genetically modified elements. Remarks were drawn on the analytical results. The fuzzy-logic based rules were shown to be applicable to improve interpretation of results and facilitate overall evaluation of the multiplex method.


Assuntos
Lógica Fuzzy , Técnicas Genéticas/estatística & dados numéricos , Organismos Geneticamente Modificados/genética , Estudos de Validação como Assunto , Algoritmos , Animais , Coleta de Dados/métodos , Coleta de Dados/estatística & dados numéricos , Interpretação Estatística de Dados , Análise em Microsséries/métodos , Análise em Microsséries/estatística & dados numéricos
20.
Environ Monit Assess ; 143(1-3): 147-59, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17985205

RESUMO

This work presents a modelling study where monthly-based climate data are used to estimate the Normalized Difference Vegetation Index (NDVI). The latter is a measure of vegetation greenness, usually derived from satellite-driven information. A model was developed to link NDVI data to rainfall and temperature measures. The test area was a 3 x 3 km grid centred to the top of Monte Pino hill (Southern Italy), for which multi-year (from 1996 to 2004) climate and satellite-derived NDVI data were available. The simulated NDVI data compared well with the remote-sensed measurements (e.g. modelling efficiency approximately 0.80), thus showing a strong linking between vegetation greenness and climate patterns in spite of the many disturbances exerted from farming. The model was used to reconstruct an extended series of monthly NDVI values for a period antecedent 1996 (1972-1995). The analysis of long-term anomalies indicated a positive trend of NDVI over time, consistent with the air temperature increase registered in the same period.


Assuntos
Clima , Ecossistema , Modelos Teóricos , Monitoramento Ambiental/métodos , Geografia , Itália , Chuva , Comunicações Via Satélite , Temperatura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA